Composite

Part:BBa_K1584036:Design

Designed by: Nu Ri Choi, Seung Meen Choi, SOOJI LEE, Brittany LEE, Hyun Min Park   Group: iGEM15_Elan_Vital_Korea   (2015-09-13)


LuxR-responsive promoter with LacZ CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101


Design Notes

J61100 has two XbaI sites, so we cut J61100 with XbaI.


Source

We used promoter(R0062), RBS(J61100) and lacZ(I732006).

References